abstract：:Low-energy ions exist widely in the natural world. People had neglected the interaction between low-energy ions and material; it was even more out of the question to study the relation of low-energy ions and the complicated organism until the biological effects of low-energy ion implantation were discovered in 1989. N...

journal_title：Applied microbiology and biotechnology

authors： Gu SB,Li SC,Feng HY,Wu Y,Yu ZL

更新日期：2008-02-01 00:00:00

abstract：:An isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as Stenotrophomonas nitritireducens by API analysis, cellular fatty acids compositions, sequencing the full 16S ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. S. nitritireducens has unique regi...

journal_title：Applied microbiology and biotechnology

authors： Yu IS,Yeom SJ,Kim HJ,Lee JK,Kim YH,Oh DK

更新日期：2008-02-01 00:00:00

abstract：:1,3-1,4-beta-D-glucanase is an important endoglycosidase in the brewing and animal feed industries. To achieve high-level expression of recombinant glucanase in Pichia pastoris, we designed sequences encoding the alpha-factor signal peptide from Saccharomyces cerevisiae and the truncated 1,3-1,4-beta-D-glucanase from ...

journal_title：Applied microbiology and biotechnology

authors： Huang H,Yang P,Luo H,Tang H,Shao N,Yuan T,Wang Y,Bai Y,Yao B

更新日期：2008-02-01 00:00:00

abstract：:A set of mutants was generated by targeted deletion of the hsdR loci of two type I restriction modification systems (RMS) identified in Bacillus licheniformis DSM13. Single as well as double knock-outs resulted in strains being readily transformable with plasmids isolated from Bacilli. Introduction of shuttle plasmids...

journal_title：Applied microbiology and biotechnology

authors： Waschkau B,Waldeck J,Wieland S,Eichstädt R,Meinhardt F

更新日期：2008-02-01 00:00:00

abstract：:High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic ac...

journal_title：Applied microbiology and biotechnology

authors： Quillaguamán J,Doan-Van T,Guzmán H,Guzmán D,Martín J,Everest A,Hatti-Kaul R

更新日期：2008-02-01 00:00:00

abstract：:Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4)....

journal_title：Applied microbiology and biotechnology

authors： Zhang W,Wang Z,Li W,Zhuang B,Qi H

更新日期：2008-02-01 00:00:00

abstract：:A moderately halophilic alkalitolerant Bacillus sp. Strain TSCVKK, with an ability to produce extracellular halophilic, alkalitolerant, surfactant, and detergent-stable alpha-amylase was isolated from soil samples obtained from a salt-manufacturing industry in Chennai. The culture conditions for higher amylase product...

journal_title：Applied microbiology and biotechnology

authors： Kiran KK,Chandra TS

更新日期：2008-01-01 00:00:00

abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to reduce manure phosphorus excretion of swine and poultry but lacks sufficient thermostability for feed pelleting and appropriate pH-activity profile for phytate hydrolysis in the stomach of animals. Previously, a thermostable mutant PhyA18 and two p...

journal_title：Applied microbiology and biotechnology

authors： Zhang W,Lei XG

更新日期：2008-01-01 00:00:00

abstract：:Grape juice contains about equal amounts of glucose and fructose, but wine strains of Saccharomyces cerevisiae ferment glucose slightly faster than fructose, leading to fructose concentrations that exceed glucose concentrations in the fermenting must. A high fructose/glucose ratio may contribute to sluggish and stuck ...

journal_title：Applied microbiology and biotechnology

authors： Berthels NJ,Cordero Otero RR,Bauer FF,Pretorius IS,Thevelein JM

更新日期：2008-01-01 00:00:00

abstract：:Because engineering of the 101.016-bp megaplasmid pKB1 of Gordonia westfalica Kb1 failed due to the absence of an effective transfer system, pKB1 was transferred by conjugation from G. westfalica Kb1 to a kanamycin-resistant mutant of Rhodococcus opacus PD630 at a frequency of about 6.2 x 10(-8) events per recipient c...

journal_title：Applied microbiology and biotechnology

authors： Bröker D,Arenskötter M,Steinbüchel A

更新日期：2008-01-01 00:00:00

abstract：:Two extracellular enzymes (MsP1 and MsP2) capable of efficient beta-carotene degradation were purified from culture supernatants of the basidiomycete Marasmius scorodonius (garlic mushroom). Under native conditions, the enzymes exhibited molecular masses of approximately 150 and approximately 120 kDa, respectively. SD...

journal_title：Applied microbiology and biotechnology

authors： Scheibner M,Hülsdau B,Zelena K,Nimtz M,de Boer L,Berger RG,Zorn H

更新日期：2008-01-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. The Cu/Zn superoxide dismutase gene (KmSOD1) of Kluyveromyces marxianus L3 was cloned and characterized. The deduced KmSod1p protein shar...

journal_title：Applied microbiology and biotechnology

authors： Raimondi S,Uccelletti D,Matteuzzi D,Pagnoni UM,Rossi M,Palleschi C

更新日期：2008-01-01 00:00:00

abstract：:A halotolerant, alkaliphilic dissimilatory Fe(III)-reducing bacterium, strain SFB, was isolated from salt flat sediments collected from Soap Lake, WA. 16S ribosomal ribonucleic acid gene sequence analysis identified strain SFB as a novel Bacillus sp. most similar to Bacillus agaradhaerens (96.7% similarity). Strain SF...

journal_title：Applied microbiology and biotechnology

authors： Pollock J,Weber KA,Lack J,Achenbach LA,Mormile MR,Coates JD

更新日期：2007-12-01 00:00:00

abstract：:The stereoselective kinetic biotransformation of thioridazine, a phenothiazine neuroleptic drug, by endophytic fungi was investigated. In general, the sulfur of lateral chain (position 2) or the sulfur of phenothiazinic ring (position 5) were oxidated yielding the major human metabolites thioridazine-2-sulfoxide and t...

journal_title：Applied microbiology and biotechnology

authors： Borges KB,Borges Wde S,Pupo MT,Bonato PS

更新日期：2007-12-01 00:00:00

abstract：:Phenylacetaldehyde reductase (PAR) from Rhodococcus sp. ST-10 is useful for chiral alcohol production because of its broad substrate specificity and high stereoselectivity. The conversion of ketones into alcohols by PAR requires the coenzyme NADH. PAR can regenerate NADH by oxidizing additional alcohols, especially 2-...

journal_title：Applied microbiology and biotechnology

authors： Makino Y,Dairi T,Itoh N

更新日期：2007-12-01 00:00:00

abstract：:After crude protein of the marine yeast strains maintained in this laboratory was estimated by the method of Kjehldahl, we found that the G7a strain which was identified to be a strain of Cryptococcus aureus according to the routine identification and molecular methods contained high level of protein and could grow on...

journal_title：Applied microbiology and biotechnology

authors： Gao L,Chi Z,Sheng J,Ni X,Wang L

更新日期：2007-12-01 00:00:00

abstract：:To seek more information on function of two-component regulatory systems (TCSs) in Streptomyces coelicolor, a dozen TCS-knockout mutants were generated, and phenotype changes were determined. One TCS (SCO5403/5404)-deleted mutant with phenotype change was obtained. Here, we report the characterization of this novel TC...

journal_title：Applied microbiology and biotechnology

authors： Lu Y,Wang W,Shu D,Zhang W,Chen L,Qin Z,Yang S,Jiang W

更新日期：2007-12-01 00:00:00

abstract：:The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. To construct an optimal C. glutamicum genome, a precise site-directed gene integration method was developed by using a pair of mutant lox sites, one a right element (RE) mutant lox site and the other a left element (LE)...

journal_title：Applied microbiology and biotechnology

authors： Suzuki N,Inui M,Yukawa H

更新日期：2007-12-01 00:00:00

abstract：:A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20-30 degrees C), agitation rate (100-300 rpm), initial pH (5.0-7.0), inoculum concentration (5-15%), and glucose conce...

journal_title：Applied microbiology and biotechnology

authors： Santos LO,Gonzales TA,Ubeda BT,Monte Alegre R

更新日期：2007-12-01 00:00:00

abstract：:Herein, we present data demonstrating that the application of initial cell culture enrichment could significantly improve mycoplasma testing methods based on the nucleic acid amplification technology (NAT) including a polymerase chain reaction (PCR)/microarray method. The results of the study using Vero cells demonstr...

journal_title：Applied microbiology and biotechnology

authors： Kong H,Volokhov DV,George J,Ikonomi P,Chandler D,Anderson C,Chizhikov V

更新日期：2007-11-01 00:00:00

abstract：:Tributyl phosphate (TBP) is a toxic organophosphorous compound widely used in nuclear fuel processing and chemical industries. Rhodopseudomonas palustris, one of the most metabolically versatile photosynthetic bacteria, is shown here to degrade TBP efficiently under photosynthetic conditions. This study shows that thi...

journal_title：Applied microbiology and biotechnology

authors： Berne C,Pignol D,Lavergne J,Garcia D

更新日期：2007-11-01 00:00:00

abstract：:A customer- and environment-friendly method for the decolorization azo dyes was developed. Azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. A new reduced nicotinamide adenine dinucleotide-dependent azoreductase of Bacillus cereus, which showed high potent...

journal_title：Applied microbiology and biotechnology

authors： Pricelius S,Held C,Murkovic M,Bozic M,Kokol V,Cavaco-Paulo A,Guebitz GM

更新日期：2007-11-01 00:00:00

abstract：:The formation and activity of an As(III)-oxidising biofilm in a bioreactor, using pozzolana as bacterial growth support, was studied for the purpose of optimising fixed-bed bioreactors for bioremediation. After 60 days of continuous functioning with an As(III)-contaminated effluent, the active biofilm was found to be ...

journal_title：Applied microbiology and biotechnology

authors： Michel C,Jean M,Coulon S,Dictor MC,Delorme F,Morin D,Garrido F

更新日期：2007-11-01 00:00:00

abstract：:Bifidobacterium bifidum NCIMB41171 carries four genes encoding different beta-galactosidases. One of them, named bbgIII, consisted of an open reading frame of 1,935 amino acid (a.a.) residues encoding a protein with a multidomain structure, commonly identified on cell wall bound enzymes, having a signal peptide, a mem...

journal_title：Applied microbiology and biotechnology

authors： Goulas TK,Goulas AK,Tzortzis G,Gibson GR

更新日期：2007-10-01 00:00:00

abstract：:Escherichia coli K-12 was cultured under anaerobic conditions to form biofilm on carbon fiber electrodes in glucose-containing medium. The anodic current increased with the development of the biofilm and depended on the glucose concentration. Cyclic voltammetric results support the presence of a redox compound(s) excr...

journal_title：Applied microbiology and biotechnology

authors： Wang YF,Tsujimura S,Cheng SS,Kano K

更新日期：2007-10-01 00:00:00

abstract：:Medium-chain-length polyhydroxyalkanoates (mcl-PHA) consisting of 3-hydroxyhexanoate (HHx), 3-hydroxyoctanoate (HO), 3-hydroxydecanoate, 3-hydroxydodecanoate, and high-content 3-hydroxytetradecanoate (HTD) was produced by knockout mutant Pseudomonas putida KT2442 termed P. putida KTOY06. When grown on 6 to14 g/L singl...

journal_title：Applied microbiology and biotechnology

authors： Liu W,Chen GQ

更新日期：2007-10-01 00:00:00

abstract：:The L-rhamnose isomerase gene (L-rhi) encoding for L-rhamnose isomerase (L-RhI) from Bacillus pallidus Y25, a facultative thermophilic bacterium, was cloned and overexpressed in Escherichia coli with a cooperation of the 6xHis sequence at a C-terminal of the protein. The open reading frame of L-rhi consisted of 1,236 ...

journal_title：Applied microbiology and biotechnology

authors： Poonperm W,Takata G,Okada H,Morimoto K,Granström TB,Izumori K

更新日期：2007-10-01 00:00:00

abstract：:Decolorization of the representative anthraquinone dye, Remazol Brilliant Blue R (RBBR) was assessed to determine the practical potential of crude recombinant dye-decolorizing peroxidase generated by Aspergillus oryzae (rDyP) in term of turnover capacity of rDyP. The turnover capacity, defined as the milligram of RBBR...

journal_title：Applied microbiology and biotechnology

authors： Shakeri M,Shoda M

更新日期：2007-09-01 00:00:00

abstract：:The biochemical oxygen demand (BOD) value is still a key parameter that can determine the level of organics, particularly the content of biodegradable organics in water. In this work, the effects of sample dilution, which should be done inevitably to get appropriate dissolved oxygen (DO) depletion, on the measurement ...

journal_title：Applied microbiology and biotechnology

authors： Seo KS,Chang HN,Park JK,Choo KH

更新日期：2007-09-01 00:00:00

abstract：:Corynebacterium glutamicum owns a citrate synthase and two methylcitrate synthases. Characterization of the isolated enzymes showed that the two methylcitrate synthases have comparable catalytic efficiency, k (cat)/K (m), as the citrate synthase with acetyl-CoA as substrate, although these enzymes are only synthesized...

journal_title：Applied microbiology and biotechnology

authors： Radmacher E,Eggeling L

更新日期：2007-09-01 00:00:00

abstract：:For the biotechnological production of L: -lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement...

journal_title：Applied microbiology and biotechnology

authors： Sindelar G,Wendisch VF

更新日期：2007-09-01 00:00:00

abstract：:The complete biodegradation of azo dye, Fast Acid Red GR, was observed under microaerophilic conditions by Shewanella decolorationis S12. Although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing ...

journal_title：Applied microbiology and biotechnology

authors： Xu M,Guo J,Sun G

更新日期：2007-09-01 00:00:00

abstract：:Effects of select electron mediators [9,10-anthraquinone-2,6-disulfonic acid disodium salt (AQDS), safranine O, resazurin, methylene blue, and humic acids] on metabolic end-products and current production from cellulose digestion by Clostridium cellulolyticum in microbial fuel cells (MFCs) were studied using capillary...

journal_title：Applied microbiology and biotechnology

authors： Sund CJ,McMasters S,Crittenden SR,Harrell LE,Sumner JJ

更新日期：2007-09-01 00:00:00

abstract：:The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis r...

journal_title：Applied microbiology and biotechnology

authors： Sakai T,Nakamura N,Umitsuki G,Nagai K,Wachi M

更新日期：2007-08-01 00:00:00

abstract：:Acid phosphatase production by recombinant Arxula adeninivorans was carried out in submerged fermentation. Using the Plackett-Burman design, three fermentation variables (pH, sucrose concentration, and peptone concentration) were identified to significantly affect acid phosphatase and biomass production, and these wer...

journal_title：Applied microbiology and biotechnology

authors： Minocha N,Kaur P,Satyanarayana T,Kunze G

更新日期：2007-08-01 00:00:00

abstract：:Autohydrogenotrophic batch growth of Ralstonia eutropha H16 was studied in a stirred-tank reactor with nitrate and nitrite as terminal electron acceptors and the sole limiting substrates. Assuming product inhibition by nitrite, saturation kinetics with the two limiting substrates and a simple switching function, which...

journal_title：Applied microbiology and biotechnology

authors： Tiemeyer A,Link H,Weuster-Botz D

更新日期：2007-08-01 00:00:00

abstract：:Sophorolipids are surface-active compounds synthesized by a selected number of yeast species. They have been known for over 40 years, but because of growing environmental awareness, they recently regained attention as biosurfactants due to their biodegradability, low ecotoxicity, and production based on renewable reso...

journal_title：Applied microbiology and biotechnology

authors： Van Bogaert IN,Saerens K,De Muynck C,Develter D,Soetaert W,Vandamme EJ

更新日期：2007-08-01 00:00:00

abstract：:With advances in the development of DNA vaccines and gene therapy, there is a growing need for plasmid DNA with high quality for fundamental research and clinical trials. In this report, a scalable automated process for large-scale preparation of plasmid is described. This process is based on alkaline lysis and can be...

journal_title：Applied microbiology and biotechnology

authors： Li X,Jin H,Wu Z,Rayner S,Yin J,Yu Y,Zhang W,He Z,Wang C,Wang B

更新日期：2007-07-01 00:00:00

abstract：:Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics and proteomics studies with other P. putida strains, the P. putida KT2440 genomic database serves as standard reference. The utility of KT2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics stu...

journal_title：Applied microbiology and biotechnology

authors： Ballerstedt H,Volkers RJ,Mars AE,Hallsworth JE,dos Santos VA,Puchalka J,van Duuren J,Eggink G,Timmis KN,de Bont JA,Wery J

更新日期：2007-07-01 00:00:00

abstract：:Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with...

journal_title：Applied microbiology and biotechnology

authors： Takeuchi Y,Nomura W,Ohdate T,Tamasu S,Masutani H,Murata K,Izawa S,Yodoi J,Inoue Y

更新日期：2007-07-01 00:00:00